PDBsum entry 7rgo

Oxidoreductase/oxidoreductase inhibitor

PDB id: 7rgo

Contents

Protein chains

157 a.a.

Ligands

NAP ×2

GOL

4SI ×2

SO4 ×2

Waters ×128

PDB id:

7rgo

Name:

Oxidoreductase/oxidoreductase inhibitor

Title:

Dfra5 complexed with NADPH and 4'-chloro-3'-(4-(2,4-diamino-6- ethylpyrimidin-5-yl)but-3-yn-2-yl)-[1,1'-biphenyl]-4-carboxamide (ucp1228)

Structure:

Dihydrofolate reductase type 5. Chain: a, b. Synonym: dihydrofolate reductase type v. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: dhfrv. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.92Å R-factor: 0.194 R-free: 0.227

Authors:

M.N.Lombardo,D.L.Wright

Key ref:

J.Krucinska et al. (2022). Structure-Guided functional studies of plasmid-Encode dihydrofolate reductases reveal a common mechanism of trimethoprim resistance in gram-Negative pathogens.. Commun biol, 5, 459. PubMed id: 35562546

Date:

15-Jul-21 Release date: 25-May-22

Protein chains

P11731  (DYR5_ECOLX) -  Dihydrofolate reductase type 5 from Escherichia coli

Seq: 157 a.a.

Struc: 157 a.a.

Enzyme reactions

• Enzyme class: E.C.1.5.1.3 - dihydrofolate reductase.
• Pathway: Folate Coenzymes
• Reaction: (6S)-5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH + H⁺

(6S)-5,6,7,8-tetrahydrofolate + NADP(+) (Bound ligand (Het Group name = NAP) corresponds exactly) = 7,8-dihydrofolate + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site