PDBsum entry 7pid

Transferase

PDB id: 7pid

Contents

Protein chains

353 a.a.

18 a.a.

Ligands

7QI

MPD

Waters ×307

PDB id:

7pid

Name:

Transferase

Title:

Protein kinase a catalytic subunit in complex with pki5-24 and en060

Structure:

Camp-dependent protein kinase catalytic subunit alpha. Chain: a. Synonym: pka c-alpha. Engineered: yes. Camp-dependent protein kinase inhibitor alpha. Chain: b. Synonym: pki-alpha,camp-dependent protein kinase inhibitor, muscle/brain isoform. Engineered: yes.

Source:

Cricetulus griseus. Chinese hamster. Organism_taxid: 10029. Organ: ovary. Gene: prkaca. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768. Synthetic: yes. Mus musculus.

Resolution:

1.50Å R-factor: 0.134 R-free: 0.161

Authors:

S.Glinca,J.M.Mueller,M.Ruf,S.Merkl

Key ref:

J.Muller et al. Magnet for the needle in haystack: "crystal structure fragment hits unlock active chemical matter using tar exploration of vast chemical spaces.. J med chem, . PubMed id: 36069712

Date:

19-Aug-21 Release date: 21-Sep-22

Protein chain

P25321  (KAPCA_CRIGR) -  cAMP-dependent protein kinase catalytic subunit alpha from Cricetulus griseus

Seq: 351 a.a.

Struc: 353 a.a.*

Protein chain

P63248  (IPKA_MOUSE) -  cAMP-dependent protein kinase inhibitor alpha from Mus musculus

Seq: 76 a.a.

Struc: 18 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.11.11 - cAMP-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site