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PDBsum entry 7k5h
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Oxidoreductase
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PDB id
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7k5h
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PDB id:
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| Name: |
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Oxidoreductase
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Title:
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1.90 a resolution structure of wt bfrb from pseudomonas aeruginosa in complex with a protein-protein interaction inhibitor km-5-66
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Structure:
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Ferroxidase. Chain: a, b, c, d, e, f, g, h, i, j, k, l. Fragment: bfrb. Engineered: yes
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Source:
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Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: atcc 15692 / pao1 / 1c / prs 101 / lmg 12228. Gene: bfrb, pa3531. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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1.90Å
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R-factor:
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0.159
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R-free:
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0.189
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Authors:
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S.Lovell,K.P.Battaile,A.Soldano,A.Punchi-Hewage,K.Meraz,J.K.Annor- Gyamfi,H.Yao,R.A.Bunce,M.Rivera
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Key ref:
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A.Soldano
et al.
(2021).
Small Molecule Inhibitors of the Bacterioferritin (BfrB)-Ferredoxin (Bfd) Complex Kill Biofilm-Embedded Pseudomonas aeruginosa Cells.
ACS Infect Dis,
7,
123-140.
PubMed id:
DOI:
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Date:
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16-Sep-20
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Release date:
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16-Dec-20
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PROCHECK
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Headers
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References
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Q9HY79
(Q9HY79_PSEAE) -
Bacterioferritin from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
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Seq:
Struc:
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158 a.a.
156 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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Enzyme class:
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E.C.1.16.3.1
- ferroxidase.
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Reaction:
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4 Fe2+ + O2 + 4 H+ = 4 Fe3+ + 2 H2O
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4
×
Fe(2+)
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+
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O2
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+
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4
×
H(+)
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=
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4
×
Fe(3+)
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+
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2
×
H2O
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Cofactor:
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Cu cation
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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DOI no:
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ACS Infect Dis
7:123-140
(2021)
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PubMed id:
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Small Molecule Inhibitors of the Bacterioferritin (BfrB)-Ferredoxin (Bfd) Complex Kill Biofilm-Embedded Pseudomonas aeruginosa Cells.
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A.Soldano,
H.Yao,
A.N.D.Punchi Hewage,
K.Meraz,
J.K.Annor-Gyamfi,
R.A.Bunce,
K.P.Battaile,
S.Lovell,
M.Rivera.
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ABSTRACT
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Bacteria depend on a well-regulated iron homeostasis to survive adverse
environments. A key component of the iron homeostasis machinery is the
compartmentalization of Fe3+ in bacterioferritin and its subsequent
mobilization as Fe2+ to satisfy metabolic requirements. In
Pseudomonas aeruginosa Fe3+ is compartmentalized in
bacterioferritin (BfrB), and its mobilization to the cytosol requires binding of
a ferredoxin (Bfd) to reduce the stored Fe3+ and release the soluble
Fe2+. Blocking the BfrB-Bfd complex in P. aeruginosa by
deletion of the bfd gene triggers an irreversible accumulation of
Fe3+ in BfrB, concomitant cytosolic iron deficiency and significant
impairment of biofilm development. Herein we report that small molecules
developed to bind BfrB at the Bfd binding site block the BfrB-Bfd complex,
inhibit the mobilization of iron from BfrB in P. aeruginosa cells,
elicit a bacteriostatic effect on planktonic cells, and are bactericidal to
cells embedded in mature biofilms.
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