PDBsum entry 7fpy

Oxidoreductase

PDB id: 7fpy

Contents

Protein chain

159 a.a.

Ligands

FOL

NAP

Metals

_MN ×3

Waters ×176

PDB id:

7fpy

Name:

Oxidoreductase

Title:

Dhfr:nadp+:fol complex at 300 k (crystal 2)

Structure:

Dihydrofolate reductase. Chain: a. Engineered: yes

Source:

Escherichia coli k-12. Organism_taxid: 83333. Strain: k12. Gene: fola, tmra, b0048, jw0047. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.12Å R-factor: 0.142 R-free: 0.170

Authors:

J.B.Greisman,K.M.Dalton,D.E.Brookner,D.R.Hekstra

Key ref:

J.B.Greisman et al. Dhfr:NADP+:fol complex at 300 k. To be published, . PubMed id: 38386706

Date:

10-Sep-22 Release date: 20-Sep-23

Protein chain

P0ABQ4  (DYR_ECOLI) -  Dihydrofolate reductase from Escherichia coli (strain K12)

Seq: 159 a.a.

Struc: 159 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.5.1.3 - dihydrofolate reductase.
• Pathway: Folate Coenzymes
• Reaction: (6S)-5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH + H⁺

(6S)-5,6,7,8-tetrahydrofolate + NADP(+) (Bound ligand (Het Group name = NAP) corresponds exactly) = 7,8-dihydrofolate (Bound ligand (Het Group name = FOL) corresponds exactly) + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site