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PDBsum entry 7f1v
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PDB id:
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Lyase
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Title:
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Crystal structure of pseudomonas putida methionine gamma-lyase q349s mutant with l-homocysteine intermediates
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Structure:
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L-methionine gamma-lyase. Chain: a, c. Synonym: mgl,homocysteine desulfhydrase,l-methioninase. Engineered: yes. Mutation: yes. L-methionine gamma-lyase. Chain: b, d. Synonym: mgl,homocysteine desulfhydrase,l-methioninase. Engineered: yes.
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Source:
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Pseudomonas putida. Arthrobacter siderocapsulatus. Organism_taxid: 303. Gene: mdea. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.25Å
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R-factor:
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0.190
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R-free:
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0.227
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Authors:
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A.Okawa,H.Handa,E.Yasuda,M.Murota,D.Kudo,T.Tamura,T.Shiba,K.Inagaki
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Key ref:
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A.Okawa
et al.
(2022).
Characterization and application of l-Methionine gamm q349s mutant enzyme with an enhanced activity toward l-Homocysteine..
J biosci bioeng,
133,
213.
PubMed id:
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Date:
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09-Jun-21
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Release date:
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20-Apr-22
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PROCHECK
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Headers
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References
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P13254
(MEGL_PSEPU) -
L-methionine gamma-lyase from Pseudomonas putida
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Seq:
Struc:
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398 a.a.
396 a.a.*
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Key: |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class 1:
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E.C.4.4.1.11
- methionine gamma-lyase.
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Reaction:
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L-methionine + H2O = methanethiol + 2-oxobutanoate + NH4+
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L-methionine
Bound ligand (Het Group name = )
matches with 88.89% similarity
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H2O
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=
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methanethiol
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+
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2-oxobutanoate
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+
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NH4(+)
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Cofactor:
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Pyridoxal 5'-phosphate
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Pyridoxal 5'-phosphate
Bound ligand (Het Group name =
7XF)
matches with 62.50% similarity
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Enzyme class 2:
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E.C.4.4.1.2
- homocysteine desulfhydrase.
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Reaction:
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L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4+ + H+
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L-homocysteine
Bound ligand (Het Group name = )
corresponds exactly
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H2O
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=
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2-oxobutanoate
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+
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hydrogen sulfide
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NH4(+)
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H(+)
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Cofactor:
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Pyridoxal 5'-phosphate
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Pyridoxal 5'-phosphate
Bound ligand (Het Group name =
7XF)
matches with 62.50% similarity
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
