PDBsum entry 7d4x

Oxidoreductase

PDB id: 7d4x

Contents

Protein chain

159 a.a.

Ligands

FOL

NAP

Waters ×68

PDB id:

7d4x

Name:

Oxidoreductase

Title:

X-ray crystal structure of e.Coli dihydrofolate reductase complexed with folate and NADP+ at ph7.0

Structure:

Dihydrofolate reductase. Chain: a. Engineered: yes

Source:

Escherichia coli (strain k12). Organism_taxid: 83333. Gene: fola, tmra, b0048, jw0047. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.60Å R-factor: 0.157 R-free: 0.189

Ensemble:

54 models

Authors:

Q.Wan,C.Dealwis

Key ref:

Q.Wan et al. (2021). Capturing the catalytic proton of dihydrofolate reduc implications for general acid-Base catalysis. Acs catalysis, 11, 5873.

Date:

24-Sep-20 Release date: 09-Jun-21

Protein chain

P0ABQ4  (DYR_ECOLI) -  Dihydrofolate reductase from Escherichia coli (strain K12)

Seq: 159 a.a.

Struc: 159 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.5.1.3 - dihydrofolate reductase.
• Pathway: Folate Coenzymes
• Reaction: (6S)-5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH + H⁺

(6S)-5,6,7,8-tetrahydrofolate + NADP(+) (Bound ligand (Het Group name = NAP) corresponds exactly) = 7,8-dihydrofolate (Bound ligand (Het Group name = FOL) corresponds exactly) + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site