PDBsum entry 7ce1

Transcription

PDB id: 7ce1

Contents

Protein chains

(+ 4 more) 331 a.a.

259 a.a.

292 a.a.

DNA/RNA

Waters ×25

PDB id:

7ce1

Name:

Transcription

Title:

Complex structure of transcription factor sghr with its cognate DNA

Structure:

Laci-type transcription factor. Chain: a, b, c, d, g, h, k, l, o, p, s, t. Engineered: yes. Promoter DNA. Chain: a, b, c, d, g, h, k, l, o, p, s, t. Engineered: yes

Source:

Agrobacterium tumefaciens a6. Organism_taxid: 1453998. Gene: sghr. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768. Agrobacterium tumefaciens. Organism_taxid: 358. Expression_system_taxid: 866768

Resolution:

3.20Å R-factor: 0.236 R-free: 0.281

Authors:

F.Z.Ye,C.Wang,X.F.Yan,L.H.Zhang,Y.G.Gao

Key ref:

F.Ye et al. (2020). Structural basis of a novel repressor, SghR, controlling Agrobacterium infection by cross-talking to plants. J Biol Chem, 295, 12290-12304. PubMed id: 32651231 DOI: 10.1074/jbc.RA120.012908

Date:

21-Jun-20 Release date: 15-Jul-20

Protein chains

A0A2I4PGE9  (A0A2I4PGE9_RHIRD) -  LacI-type transcription factor (Fragment) from Agrobacterium tumefaciens A6

Seq: 350 a.a.

Struc: 331 a.a.

Protein chain

A0A2I4PGE9  (A0A2I4PGE9_RHIRD) -  LacI-type transcription factor (Fragment) from Agrobacterium tumefaciens A6

Seq: 350 a.a.

Struc: 259 a.a.

Protein chain

A0A2I4PGE9  (A0A2I4PGE9_RHIRD) -  LacI-type transcription factor (Fragment) from Agrobacterium tumefaciens A6

Seq: 350 a.a.

Struc: 292 a.a.

DNA/RNA chains

T-A-T-C-T-G-C-A-A-C-G-T-T-G-C-A-G-A 18 bases

T-A-T-C-T-G-C-A-A-C-G-T-T-G-C-A-G-A 18 bases

T-A-T-C-T-G-C-A-A-C-G-T-T-G-C-A-G-A 18 bases

T-A-T-C-T-G-C-A-A-C-G-T-T-G-C-A-G-A 18 bases

A-T-C-T-G-C-A-A-C-G-T-T-G-C-A-G-A 17 bases

A-T-C-T-G-C-A-A-C-G-T-T-G-C-A-G-A 17 bases

A-T-C-T-G-C-A-A-C-G-T-T-G-C-A-G-A 17 bases

A-T-C-T-G-C-A-A-C-G-T-T-G-C-A-G-A 17 bases

T-A-T-C-T-G-C-A-A-C-G-T-T-G-C-A-G-A 18 bases

A-T-C-T-G-C-A-A-C-G-T-T-G-C-A-G-A 17 bases

T-C-T-G-C-A-A-C-G-T-T-G-C-A 14 bases

A-T-C-T-G-C-A-A-C-G-T-T-G-C 14 bases

DOI no: 10.1074/jbc.RA120.012908

J Biol Chem 295:12290-12304 (2020)

PubMed id: 32651231

Structural basis of a novel repressor, SghR, controlling Agrobacterium infection by cross-talking to plants.

F.Ye, C.Wang, Q.Fu, X.F.Yan, S.R.Bharath, A.Casanas, M.Wang, H.Song, L.H.Zhang, Y.G.Gao.

ABSTRACT

Agrobacterium tumefaciens infects various plants and causes crown gall diseases involving temporal expression of virulence factors. SghA is a newly identified virulence factor enzymatically releasing salicylic acid from its glucoside conjugate and controlling plant tumor development. Here, we report the structural basis of SghR, a LacI-type transcription factor highly conserved in Rhizobiaceae family, regulating the expression of SghA and involved in tumorigenesis. We identified and characterized the binding site of SghR on the promoter region of sghA and then determined the crystal structures of apo-SghR, SghR complexed with its operator DNA, and ligand sucrose, respectively. These results provide detailed insights into how SghR recognizes its cognate DNA and shed a mechanistic light on how sucrose attenuates the affinity of SghR with DNA to modulate the expression of SghA. Given the important role of SghR in mediating the signaling cross-talk during Agrobacterium infection, our results pave the way for structure-based inducer analog design, which has potential applications for agricultural industry.