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PDBsum entry 7ce1
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Transcription
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PDB id
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7ce1
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Contents |
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(+ 4 more)
331 a.a.
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259 a.a.
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292 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural basis of a novel repressor, Sghr, Controlling agrobacterium infection by cross-Talking to plants.
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Authors
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F.Ye,
C.Wang,
Q.Fu,
X.F.Yan,
S.R.Bharath,
A.Casanas,
M.Wang,
H.Song,
L.H.Zhang,
Y.G.Gao.
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Ref.
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J Biol Chem, 2020,
295,
12290-12304.
[DOI no: ]
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PubMed id
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Abstract
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Agrobacterium tumefaciens infects various plants and causes crown gall
diseases involving temporal expression of virulence factors. SghA is a newly
identified virulence factor enzymatically releasing salicylic acid from its
glucoside conjugate and controlling plant tumor development. Here, we report the
structural basis of SghR, a LacI-type transcription factor highly conserved in
Rhizobiaceae family, regulating the expression of SghA and involved in
tumorigenesis. We identified and characterized the binding site of SghR on the
promoter region of sghA and then determined the crystal structures of
apo-SghR, SghR complexed with its operator DNA, and ligand sucrose,
respectively. These results provide detailed insights into how SghR recognizes
its cognate DNA and shed a mechanistic light on how sucrose attenuates the
affinity of SghR with DNA to modulate the expression of SghA. Given the
important role of SghR in mediating the signaling cross-talk during
Agrobacterium infection, our results pave the way for structure-based
inducer analog design, which has potential applications for agricultural
industry.
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