PDBsum entry 7ap2

Ligase

PDB id: 7ap2

Contents

Protein chain

843 a.a.

Ligands

EDO ×2

RRW

Metals

_MG

_ZN

Waters ×213

PDB id:

7ap2

Name:

Ligase

Title:

Neisseria gonorrhoeae leucyl-tRNA synthetase in complex with compound leus7hmdda

Structure:

Leucine--tRNA ligase. Chain: a. Fragment: leucyl-tRNA synthetase. Synonym: leucyl-tRNA synthetase,leurs. Engineered: yes. Mutation: yes

Source:

Neisseria gonorrhoeae. Organism_taxid: 485. Gene: leus, vt05_02036, whoo_00006, whoo_00455. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.25Å R-factor: 0.190 R-free: 0.235

Authors:

L.Pang,S.De Graef,S.V.Strelkov,S.D.Weeks

Key ref:

B.Zhang et al. (2020). Synthesis and biological evaluation of 1,3-Dideazapurine-Like 7-Amino-5-Hydroxymethyl-Benzim ribonucleoside analogues as aminoacyl-Trna synthetase inhibitors.. Molecules, 25, . PubMed id: 33081246

Date:

15-Oct-20 Release date: 28-Oct-20

Protein chain

Q5FAJ3  (SYL_NEIG1) -  Leucine--tRNA ligase from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)

Seq: 876 a.a.

Struc: 843 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.6.1.1.4 - leucine--tRNA ligase.
• Reaction: tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site