PDBsum entry 7adc

Transcription

PDB id: 7adc

Contents

Protein chains

(+ 0 more) 417 a.a.

495 a.a.

235 a.a.

321 a.a.

79 a.a.

1340 a.a.

1358 a.a.

DNA/RNA

Ligands

ADP ×5

BEF ×5

Metals

_MG ×6

_ZN ×2

Waters ×15

PDB id:

7adc

Name:

Transcription

Title:

Transcription termination intermediate complex 3 delta nusg

Structure:

Transcription termination factor rho. Chain: f, a, b, c, d, e. Synonym: atp-dependent helicase rho. Engineered: yes. Transcription termination/antitermination protein nusa. Chain: a. Engineered: yes. DNA-directed RNA polymerase subunit alpha. Chain: u, v.

Source:

Escherichia coli. Organism_taxid: 562. Gene: rho, ac789_1c41660, acn002_3874, el75_4398, el79_4648, el80_4555. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: nusa, a6581_13525, a8c65_02280, a9819_18195, a9r57_12710, a9x72_02950, ac789_1c35390, acn002_3262, acn68_12470, acn81_18560, acu57_14555, acu90_11675, aj318_05130, am270_15620, am464_08470,

Authors:

N.Said,T.Hilal,B.Loll,C.M.Wahl

Key ref:

n.said et al. (2021). Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase rho. Science, 371, . PubMed id: 33243850

Date:

14-Sep-20 Release date: 25-Nov-20

Protein chains

P0AG30  (RHO_ECOLI) -  Transcription termination factor Rho from Escherichia coli (strain K12)

Seq: 419 a.a.

Struc: 417 a.a.

Protein chain

P0AFF6  (NUSA_ECOLI) -  Transcription termination/antitermination protein NusA from Escherichia coli (strain K12)

Seq: 495 a.a.

Struc: 495 a.a.

Protein chain

P0A7Z4  (RPOA_ECOLI) -  DNA-directed RNA polymerase subunit alpha from Escherichia coli (strain K12)

Seq: 329 a.a.

Struc: 235 a.a.

Protein chain

P0A7Z4  (RPOA_ECOLI) -  DNA-directed RNA polymerase subunit alpha from Escherichia coli (strain K12)

Seq: 329 a.a.

Struc: 321 a.a.

Protein chain

P0A800  (RPOZ_ECOLI) -  DNA-directed RNA polymerase subunit omega from Escherichia coli (strain K12)

Seq: 91 a.a.

Struc: 79 a.a.

Protein chain

P0A8V2  (RPOB_ECOLI) -  DNA-directed RNA polymerase subunit beta from Escherichia coli (strain K12)

Seq: 1342 a.a.

Struc: 1340 a.a.

Protein chain

P0A8T7  (RPOC_ECOLI) -  DNA-directed RNA polymerase subunit beta' from Escherichia coli (strain K12)

Seq: 1407 a.a.

Struc: 1358 a.a.

DNA/RNA chains

C-C-G-A-G-C-A-G-C-T-A-C-T-T-G-T-G-A-G-C-G-G-A-T-A 25 bases

T-A-T-C-C-G-C-T-C-A-C-A-A-T-G-C-C-A-C-A-C-G-C-G-C-T-G-C-T-C-G-G-C-C 34 bases

C-A-C-A-U-U-C-C-A-A-C-C-A-C-A-C-C-U-G-G-C-G-U-G-U-G-G-C 28 bases

Enzyme reactions

• Enzyme class 1: Chains U, V, W, X, Y: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 2: Chains f, a, b, c, d, e: E.C.3.6.4.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Science 371: (2021)

PubMed id: 33243850

Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase rho.

n.said, t.hilal, b.loll, m.c.wahl.

ABSTRACT

No abstract given.