PDBsum entry 7a5t

Hydrolase

PDB id: 7a5t

Contents

Protein chain

266 a.a.

Ligands

GOL ×9

PO4

Waters ×207

PDB id:

7a5t

Name:

Hydrolase

Title:

Crystal structure of a55e mutant of blac from mycobacterium tuberculosis

Structure:

Beta-lactamase. Chain: a. Engineered: yes. Mutation: yes

Source:

Mycobacterium tuberculosis. Organism_taxid: 1773. Gene: blac, ers027646_02769. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.40Å R-factor: 0.149 R-free: 0.177

Authors:

A.Chikunova,M.U.Ahmad,A.Perrakis,M.Ubbink

Key ref:

I.Van alen et al. (2021). The g132s mutation enhances the resistance of mycobac tuberculosis beta-Lactamase against sulbactam.. Biochemistry, 60, 2236. PubMed id: 34250791

Date:

21-Aug-20 Release date: 07-Jul-21

Protein chain

P9WKD3  (BLAC_MYCTU) -  Beta-lactamase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 307 a.a.

Struc: 266 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)