PDBsum entry 6yac

Photosynthesis

PDB id: 6yac

Contents

Protein chains

743 a.a.

733 a.a.

80 a.a.

143 a.a.

66 a.a.

154 a.a.

97 a.a.

88 a.a.

31 a.a.

42 a.a.

81 a.a.

157 a.a.

193 a.a.

208 a.a.

221 a.a.

198 a.a.

97 a.a.

Ligands

CL0

CLA ×141

PQN ×2

SF4 ×3

BCR ×27

LHG ×8

LMT ×6

LMG ×19

DGD ×8

LUT ×7

CHL

XAT ×2

C7Z

FES

Metals

_CA

Waters ×56

PDB id:

6yac

Name:

Photosynthesis

Title:

Plant psi-ferredoxin supercomplex

Structure:

Photosystem i p700 chlorophyll a apoprotein a1. Chain: a. Synonym: psi-a,psaa. Photosystem i p700 chlorophyll a apoprotein a2. Chain: b. Synonym: psi-b,psab. Photosystem i iron-sulfur center. Chain: c. Synonym: 9 kda polypeptide,psi-c,photosystem i subunit vii,psac.

Source:

Pisum sativum. Pea. Organism_taxid: 3888. Organism_taxid: 3888

Authors:

I.Caspy,N.Nelson,Y.Shkolnisky,D.Klaiman,A.Sheinker

Key ref:

I.Caspy et al. (2020). The structure of a triple complex of plant photosystem I with ferredoxin and plastocyanin. Nat Plants, 6, 1300-1305. PubMed id: 33020607 DOI: 10.1038/s41477-020-00779-9

Date:

12-Mar-20 Release date: 30-Sep-20

Protein chain

A0A0F6NFW5  (A0A0F6NFW5_PEA) -  Photosystem I P700 chlorophyll a apoprotein A1 from Pisum sativum

Seq: 758 a.a.

Struc: 743 a.a.

Protein chain

A0A0F6NGI2  (A0A0F6NGI2_PEA) -  Photosystem I P700 chlorophyll a apoprotein A2 from Pisum sativum

Seq: 734 a.a.

Struc: 733 a.a.

Protein chain

P10793  (PSAC_PEA) -  Photosystem I iron-sulfur center from Pisum sativum

Seq: 81 a.a.

Struc: 80 a.a.

Protein chain

E1C9K8  (E1C9K8_PEA) -  Photosystem I reaction center subunit II, chloroplastic from Pisum sativum

Seq: 138 a.a.

Struc: 143 a.a.*

Protein chain

E1C9K6  (E1C9K6_PEA) -  Photosystem I reaction center subunit IV from Pisum sativum

Seq: 64 a.a.

Struc: 66 a.a.*

Protein chain

A0A0M3KL12  (A0A0M3KL12_PEA) -  Photosystem I reaction center subunit III from Pisum sativum

Seq: 154 a.a.

Struc: 154 a.a.*

Protein chain

No UniProt id for this chain

Struc: 97 a.a.

Protein chain

A0A0M3KL10  (A0A0M3KL10_PEA) -  Photosystem I reaction center subunit VI from Pisum sativum

Seq: 90 a.a.

Struc: 88 a.a.*

Protein chain

P17227  (PSAI_PEA) -  Photosystem I reaction center subunit VIII from Pisum sativum

Seq: 40 a.a.

Struc: 31 a.a.

Protein chain

D5MAL3  (D5MAL3_PEA) -  Photosystem I reaction center subunit IX from Pisum sativum

Seq: 42 a.a.

Struc: 42 a.a.*

Protein chain

E1C9L3  (E1C9L3_PEA) -  Photosystem I reaction center subunit psaK, chloroplastic from Pisum sativum

Seq: 84 a.a.

Struc: 81 a.a.*

Protein chain

E1C9L1  (E1C9L1_PEA) -  Photosystem I reaction center subunit XI, chloroplastic from Pisum sativum

Seq: 161 a.a.

Struc: 157 a.a.*

Protein chain

No UniProt id for this chain

Struc: 193 a.a.

Protein chain

Q41038  (Q41038_PEA) -  Chlorophyll a-b binding protein, chloroplastic from Pisum sativum

Seq: 269 a.a.

Struc: 208 a.a.

Protein chain

Q32904  (CB23_PEA) -  Chlorophyll a-b binding protein 3, chloroplastic from Pisum sativum

Seq: 275 a.a.

Struc: 221 a.a.

Protein chain

Q9SQL2  (CB24_PEA) -  Chlorophyll a-b binding protein P4, chloroplastic from Pisum sativum

Seq: 252 a.a.

Struc: 198 a.a.*

Protein chain

P09911  (FER1_PEA) -  Ferredoxin-1, chloroplastic from Pisum sativum

Seq: 149 a.a.

Struc: 97 a.a.

* PDB and UniProt seqs differ at 38 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C: E.C.1.97.1.12 - photosystem I.
• Reaction: reduced [plastocyanin] + hnu + oxidized [2Fe-2S]-[ferredoxin] = oxidized [plastocyanin] + reduced [2Fe-2S]-[ferredoxin]

DOI no: 10.1038/s41477-020-00779-9

Nat Plants 6:1300-1305 (2020)

PubMed id: 33020607

The structure of a triple complex of plant photosystem I with ferredoxin and plastocyanin.

I.Caspy, A.Borovikova-Sheinker, D.Klaiman, Y.Shkolnisky, N.Nelson.

ABSTRACT

The ability of photosynthetic organisms to use sunlight as a sole source of energy is endowed by two large membrane complexes-photosystem I (PSI) and photosystem II (PSII). PSI and PSII are the fundamental components of oxygenic photosynthesis, providing oxygen, food and an energy source for most living organisms on Earth. Currently, high-resolution crystal structures of these complexes from various organisms are available. The crystal structures of megadalton complexes have revealed excitation transfer and electron-transport pathways within the various complexes. PSI is defined as plastocyanin-ferredoxin oxidoreductase but a high-resolution structure of the entire triple supercomplex is not available. Here, using a new cryo-electron microscopy technique, we solve the structure of native plant PSI in complex with its electron donor plastocyanin and the electron acceptor ferredoxin. We reveal all of the contact sites and the modes of interaction between the interacting electron carriers and PSI.