PDBsum entry 6tjh

De novo protein

PDB id: 6tjh

Contents

Protein chains

359 a.a.

Ligands

SO4 ×14

GOL

Waters ×1011

PDB id:

6tjh

Name:

De novo protein

Title:

Crystal structure of the computationally designed cake9 protein

Structure:

Cake9. Chain: a, b. Engineered: yes

Source:

Synthetic construct. Organism_taxid: 32630. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.43Å R-factor: 0.166 R-free: 0.193

Authors:

B.Mylemans,I.Laier,H.Noguchi,A.R.D.Voet

Key ref:

B.Mylemans et al. (2021). Structural plasticity of a designer protein sheds light on β-propeller protein evolution. FEBS J, 288, 530-545. PubMed id: 32343866 DOI: 10.1111/febs.15347

Date:

26-Nov-19 Release date: 06-May-20

Protein chains

No UniProt id for this chain

Struc: 359 a.a.

DOI no: 10.1111/febs.15347

FEBS J 288:530-545 (2021)

PubMed id: 32343866

Structural plasticity of a designer protein sheds light on β-propeller protein evolution.

B.Mylemans, I.Laier, K.Kamata, S.Akashi, H.Noguchi, J.R.H.Tame, A.R.D.Voet.

ABSTRACT

β-propeller proteins are common in nature, where they are observed to adopt 4- to 10-fold internal rotational pseudo-symmetry. This size diversity can be explained by the evolutionary process of gene duplication and fusion. In this study, we investigated a distorted β-propeller protein, an apparent intermediate between two symmetries. From this template, we created a perfectly symmetric 9-bladed β-propeller named Cake, using computational design and ancestral sequence reconstruction. The designed repeat sequence was found to be capable of generating both 8-fold and 9-fold propellers which are highly stable. Cake variants with 2-10 identical copies of the repeat sequence were characterised by X-ray crystallography and in solution. They were found to be highly stable, and to self-assemble into 8- or 9-fold symmetrical propellers. These findings show that the β-propeller fold allows sufficient structural plasticity to permit a given blade to assemble different forms, a transition from even to odd changes in blade number, and provide a potential explanation for the wide diversity of repeat numbers observed in natural propeller proteins. DATABASE: Structural data are available in Protein Data Bank database under the accession numbers 6TJB, 6TJC, 6TJD, 6TJE, 6TJF, 6TJG, 6TJH and 6TJI.