PDBsum entry 6prm

Hydrolase

PDB id: 6prm

Contents

Protein chains

447 a.a.

Metals

_CA ×4

Waters ×104

PDB id:

6prm

Name:

Hydrolase

Title:

Crystal structure of apo pss1_19b

Structure:

Exo-4s-kappa carrageenan s1 sulfatase. Chain: a, b, c, d. Engineered: yes

Source:

Pseudoalteromonas fuliginea. Organism_taxid: 1872678. Strain: ps47. Gene: eu509_08890. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.50Å R-factor: 0.239 R-free: 0.273

Authors:

A.G.Hettle,A.B.Boraston

Key ref:

A.G.Hettle et al. (2019). Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species. Commun Biol, 2, 474. PubMed id: 31886414 DOI: 10.1038/s42003-019-0721-y

Date:

10-Jul-19 Release date: 25-Sep-19

Protein chains

No UniProt id for this chain

Struc: 447 a.a.

DOI no: 10.1038/s42003-019-0721-y

Commun Biol 2:474 (2019)

PubMed id: 31886414

Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species.

A.G.Hettle, J.K.Hobbs, B.Pluvinage, C.Vickers, K.T.Abe, O.Salama-Alber, B.E.McGuire, J.H.Hehemann, J.P.M.Hui, F.Berrue, A.Banskota, J.Zhang, E.M.Bottos, J.Van Hamme, A.B.Boraston.

ABSTRACT

Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.