PDBsum entry 6jcs

Virus

PDB id: 6jcs

Contents

Protein chains

516 a.a.

97 a.a.

PDB id:

6jcs

Name:

Virus

Title:

Aav5 in complex with aavr

Structure:

Capsid protein. Chain: a. Dyslexia-associated protein kiaa0319-like protein. Chain: r. Fragment: pkd1. Synonym: adeno-associated virus receptor,aavr. Engineered: yes

Source:

Adeno-associated virus - 5. Organism_taxid: 82300. Homo sapiens. Human. Organism_taxid: 9606. Gene: kiaa0319l, aavr, kiaa1837, pp791. Expressed in: escherichia coli. Expression_system_taxid: 562

Authors:

Z.Lou,R.Zhang

Key ref:

R.Zhang et al. (2019). Divergent engagements between adeno-associated viruses with their cellular receptor AAVR. Nat Commun, 10, 3760. PubMed id: 31434885 DOI: 10.1038/s41467-019-11668-x

Date:

30-Jan-19 Release date: 14-Aug-19

Protein chain

Q9YIJ1  (Q9YIJ1_9VIRU) -  Capsid protein from adeno-associated virus 5

Seq: 724 a.a.

Struc: 516 a.a.

Protein chain

Q8IZA0  (K319L_HUMAN) -  Dyslexia-associated protein KIAA0319-like protein from Homo sapiens

Seq: 1049 a.a.

Struc: 97 a.a.

DOI no: 10.1038/s41467-019-11668-x

Nat Commun 10:3760 (2019)

PubMed id: 31434885

Divergent engagements between adeno-associated viruses with their cellular receptor AAVR.

R.Zhang, G.Xu, L.Cao, Z.Sun, Y.He, M.Cui, Y.Sun, S.Li, H.Li, L.Qin, M.Hu, Z.Yuan, Z.Rao, W.Ding, Z.Rao, Z.Lou.

ABSTRACT

Adeno-associated virus (AAV) receptor (AAVR) is an essential receptor for the entry of multiple AAV serotypes with divergent rules; however, the mechanism remains unclear. Here, we determine the structures of the AAV1-AAVR and AAV5-AAVR complexes, revealing the molecular details by which PKD1 recognizes AAV5 and PKD2 is solely engaged with AAV1. PKD2 lies on the plateau region of the AAV1 capsid. However, the AAV5-AAVR interface is strikingly different, in which PKD1 is bound at the opposite side of the spike of the AAV5 capsid than the PKD2-interacting region of AAV1. Residues in strands F/G and the CD loop of PKD1 interact directly with AAV5, whereas residues in strands B/C/E and the BC loop of PKD2 make contact with AAV1. These findings further the understanding of the distinct mechanisms by which AAVR recognizes various AAV serotypes and provide an example of a single receptor engaging multiple viral serotypes with divergent rules.