PDBsum entry 6d4c

Oxidoreductase

PDB id: 6d4c

Contents

Protein chains

361 a.a.

Ligands

NAD ×2

AZI ×2

Metals

_CL ×2

Waters ×1055

PDB id:

6d4c

Name:

Oxidoreductase

Title:

Crystal structure of candida boidinii formate dehydrogenase v123g mutant complexed with NAD+ and azide

Structure:

Formate dehydrogenase. Chain: a, b. Synonym: fdh,NAD-dependent formate dehydrogenase. Engineered: yes. Mutation: yes

Source:

Candida boidinii. Yeast. Organism_taxid: 5477. Gene: fdh. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768

Resolution:

1.45Å R-factor: 0.161 R-free: 0.185

Authors:

Q.Guo,H.Ye,L.Gakhar,C.M.Cheatum,A.Kohen

Key ref:

Q.Guo et al. Crystal structure of candida boidinii formate dehydro V123g mutant complexed with NAD+ and azide. To be published, .

Date:

17-Apr-18 Release date: 24-Apr-19

Protein chains

A0A0A1EQY0  (A0A0A1EQY0_CANBO) -  Formate dehydrogenase from Candida boidinii

Seq: 364 a.a.

Struc: 361 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.17.1.9 - formate dehydrogenase.
• Reaction: formate + NAD⁺ = CO2 + NADH

formate + NAD(+) (Bound ligand (Het Group name = NAD) corresponds exactly) = CO2 + NADH

• Cofactor: Flavin; Iron-sulfur; Mo cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site