PDBsum entry 5znh

Oxidoreductase

PDB id: 5znh

Contents

Protein chain

314 a.a.

Ligands

MCT

PEG

EDO

Metals

_CA

_FE

Waters ×33

PDB id:

5znh

Name:

Oxidoreductase

Title:

Catechol 2,3-dioxygenase with 4-methyl catechol from diaphorobacter sp ds2

Structure:

Catechol 2,3-dioxygenase, extradiol protein. Chain: a. Engineered: yes

Source:

Diaphorobacter sp. Ds2. Organism_taxid: 1302548. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768

Resolution:

2.40Å R-factor: 0.201 R-free: 0.239

Authors:

K.Mishra,C.K.Arya,R.Subramaniyan,G.Ramanathan

Key ref:

K.Mishra et al. Catechol 2,3-Dioxygenase with 4-Methyl catechol from diaphorobacter sp ds2. To be published, .

Date:

09-Apr-18 Release date: 17-Apr-19

Protein chain

A0A4V8GZK8  (A0A4V8GZK8_9BURK) -  Metapyrocatechase from Diaphorobacter sp. DS2

Seq: 314 a.a.

Struc: 314 a.a.

Enzyme reactions

• Enzyme class: E.C.1.13.11.2 - catechol 2,3-dioxygenase.
• Reaction: catechol + O2 = (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H⁺

catechol (Bound ligand (Het Group name = MCT) matches with 88.89% similarity) + O2 = (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H(+)

• Cofactor: Fe(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site