PDBsum entry 5l1l

Transferase/DNA

PDB id: 5l1l

Contents

Protein chain

430 a.a.

DNA/RNA

Ligands

0KX

GOL ×3

Metals

_MG ×2

Waters ×529

PDB id:

5l1l

Name:

Transferase/DNA

Title:

Postinsertion complex of human DNA polymerase eta bypassing an o6- methyl-2'-deoxyguanosine : dt site

Structure:

DNA polymerase eta. Chain: a. Synonym: rad30 homolog a,xeroderma pigmentosum variant type protein. Engineered: yes. DNA (5'-d( Cp Ap Tp Gp (6Og)p Tp Gp Ap Cp Gp Cp T)-3'). Chain: t. Engineered: yes. DNA (5'-d( Ap Gp Cp Gp Tp Cp Ap T)-3'). Chain: p.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: polh, rad30, rad30a, xpv. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Resolution:

1.62Å R-factor: 0.159 R-free: 0.193

Authors:

A.Patra,M.Egli

Key ref:

A.Patra et al. (2016). Mechanisms of Insertion of dCTP and dTTP Opposite the DNA Lesion O6-Methyl-2'-deoxyguanosine by Human DNA Polymerase η. J Biol Chem, 291, 24304-24313. PubMed id: 27694439 DOI: 10.1074/jbc.M116.755462

Date:

29-Jul-16 Release date: 05-Oct-16

Protein chain

Q9Y253  (POLH_HUMAN) -  DNA polymerase eta from Homo sapiens

Seq: 713 a.a.

Struc: 430 a.a.

DNA/RNA chains

C-A-T-G-6OG-T-G-A-C-G-C-T 12 bases

A-G-C-G-T-C-A-T 8 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1074/jbc.M116.755462

J Biol Chem 291:24304-24313 (2016)

PubMed id: 27694439

Mechanisms of Insertion of dCTP and dTTP Opposite the DNA Lesion O6-Methyl-2'-deoxyguanosine by Human DNA Polymerase η.

A.Patra, Q.Zhang, F.P.Guengerich, M.Egli.

ABSTRACT

No abstract given.