PDBsum entry 5kl1

RNA binding protein/RNA

PDB id: 5kl1

Contents

Protein chains

328 a.a.

70 a.a.

DNA/RNA

Metals

_ZN ×2

PDB id:

5kl1

Name:

RNA binding protein/RNA

Title:

Crystal structure of the pumilio-nos-hunchback RNA complex

Structure:

Maternal protein pumilio. Chain: a. Fragment: unp residues 1091-1426. Engineered: yes. Protein nanos. Chain: b. Fragment: unp residues 289-401. Engineered: yes. RNA (5'-r( Ap Ap Ap Up Up Gp Up Ap Cp Ap Up A)-3').

Source:

Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: pum, cg9755. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: nos, cg5637. Synthetic: yes. Organism_taxid: 7227

Resolution:

3.70Å R-factor: 0.268 R-free: 0.300

Authors:

C.Qiu,T.M.T.Hall

Key ref:

C.A.Weidmann et al. Drosophila nanos acts as a molecular clamp that modul the RNA-Binding and repression activities of pumilio. Elife, . PubMed id: 27482653

Date:

23-Jun-16 Release date: 17-Aug-16

Protein chain

P25822  (PUM_DROME) -  Maternal protein pumilio from Drosophila melanogaster

Seq: 1533 a.a.

Struc: 328 a.a.

Protein chain

P25724  (NANOS_DROME) -  Protein nanos from Drosophila melanogaster

Seq: 401 a.a.

Struc: 70 a.a.

DNA/RNA chain

A-A-A-U-U-G-U-A-C-A-U-A 12 bases