PDBsum entry 5kjs

Transferase

PDB id: 5kjs

Contents

Protein chain

429 a.a.

Waters ×199

PDB id:

5kjs

Name:

Transferase

Title:

Crystal structure of arabidopsis thaliana hct

Structure:

Shikimate o-hydroxycinnamoyltransferase. Chain: a. Synonym: hydroxycinnamoyl transferase,hydroxycinnamoyl-coenzyme a shikimate/quinate hydroxycinnamoyltransferase. Engineered: yes

Source:

Arabidopsis thaliana. Mouse-ear cress. Organism_taxid: 3702. Gene: hst, hct, at5g48930, k19e20.4. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.20Å R-factor: 0.183 R-free: 0.241

Authors:

O.Levsh,Y.C.Chiang,C.Tung,J.P.Noel,Y.Wang,J.K.Weng

Key ref:

O.Levsh et al. (2016). Dynamic Conformational States Dictate Selectivity toward the Native Substrate in a Substrate-Permissive Acyltransferase. Biochemistry, 55, 6314-6326. PubMed id: 27805809 DOI: 10.1021/acs.biochem.6b00887

Date:

20-Jun-16 Release date: 02-Nov-16

Protein chain

Q9FI78  (HST_ARATH) -  Shikimate O-hydroxycinnamoyltransferase from Arabidopsis thaliana

Seq: 433 a.a.

Struc: 429 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.1.133 - shikimate O-hydroxycinnamoyltransferase.
• Reaction: shikimate + 4-coumaroyl-CoA = trans-4-coumaroylshikimate + CoA

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1021/acs.biochem.6b00887

Biochemistry 55:6314-6326 (2016)

PubMed id: 27805809

Dynamic Conformational States Dictate Selectivity toward the Native Substrate in a Substrate-Permissive Acyltransferase.

O.Levsh, Y.C.Chiang, C.F.Tung, J.P.Noel, Y.Wang, J.K.Weng.

ABSTRACT

No abstract given.