PDBsum entry 5e8q

Oxidoreductase

PDB id: 5e8q

Contents

Protein chains

159 a.a.

Ligands

FOL ×2

IPA

Metals

_CL ×4

_CA

Waters ×324

PDB id:

5e8q

Name:

Oxidoreductase

Title:

Crystal structure of dhfr in 20% isopropanol

Structure:

Dihydrofolate reductase. Chain: b, a. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: fola, aa953_06220, abe89_23390, abe90_01590, abe91_11465, abe92_27580, abe93_03540, abe94_26505, abe95_19105, ac789_1c00510, acm16_01115, acm17_00910, acm18_00225, acm19_01115, acm20_00925, acm21_22820, bn1008_2275, econih1_00280, ecs0051, el75_3711, el79_3822, el80_3768, hw43_03985, iy32_08385, jd73_13725, nmeco18_25965, pd07_16380, pu06_14795, pu15_01135, pu21_08285, pu33_00085, pu38_11910, pu53_21230, pu69_06270, pu71_09850,

Resolution:

1.80Å R-factor: 0.200 R-free: 0.253

Authors:

M.J.Cuneo,P.K.Agarwal

Key ref:

J.M.Borrguero et al. Solvent conditions control enzyme dynamics and cataly. To be published, .

Date:

14-Oct-15 Release date: 21-Sep-16

Protein chains

P0ABQ4  (DYR_ECOLI) -  Dihydrofolate reductase from Escherichia coli (strain K12)

Seq: 159 a.a.

Struc: 159 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.5.1.3 - dihydrofolate reductase.
• Pathway: Folate Coenzymes
• Reaction: (6S)-5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH + H⁺

(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate (Bound ligand (Het Group name = FOL) corresponds exactly) + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site