PDBsum entry 5e2g

Hydrolase

PDB id: 5e2g

Contents

Protein chains

349 a.a.

Ligands

SCN ×6

ACY ×2

Waters ×702

PDB id:

5e2g

Name:

Hydrolase

Title:

Crystal structure of d-alanine carboxypeptidase ampc from burkholderia cenocepacia

Structure:

Beta-lactamase. Chain: a, b. Fragment: residues 31-388. Engineered: yes

Source:

Burkholderia cenocepacia. Organism_taxid: 216591. Strain: atcc baa-245 / dsm 16553 / lmg 16656 / nctc 13227 / j2315 / cf5610. Gene: ampc, bcas0156. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.65Å R-factor: 0.153 R-free: 0.189

Authors:

Y.Kim,G.Joachimiak,M.Endres,G.Babnigg,A.Joachimiak,Midwest Center For Structural Genomics (Mcsg)

Key ref:

Y.Kim et al. Crystal structure of d-Alanine carboxypeptidase ampc burkholderia cenocepacia. To be published, .

Date:

01-Oct-15 Release date: 14-Oct-15

Protein chains

B4EPS2  (B4EPS2_BURCJ) -  Beta-lactamase from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

Seq: 389 a.a.

Struc: 349 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)