PDBsum entry 5e12

Transferase

PDB id: 5e12

Contents

Protein chains

202 a.a.

189 a.a.

Ligands

FLC

Waters ×141

PDB id:

5e12

Name:

Transferase

Title:

Crystal structure of pasta domains 2, 3 and 4 of mycobacterium tuberculosis protein kinase b

Structure:

Serine/threonine-protein kinase pknb. Chain: a, b. Fragment: unp residues 423-626. Engineered: yes

Source:

Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: atcc 25618 / h37rv. Gene: pknb, rv0014c, mtcy10h4.14c. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.21Å R-factor: 0.207 R-free: 0.249

Authors:

D.M.Prigozhin,Tb Structural Genomics Consortium (Tbsgc)

Key ref:

D.M.Prigozhin et al. (2016). Structural and Genetic Analyses of the Mycobacterium tuberculosis Protein Kinase B Sensor Domain Identify a Potential Ligand-binding Site. J Biol Chem, 291, 22961-22969. PubMed id: 27601474 DOI: 10.1074/jbc.M116.731760

Date:

29-Sep-15 Release date: 14-Sep-16

Protein chain

P9WI81  (PKNB_MYCTU) -  Serine/threonine-protein kinase PknB from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 626 a.a.

Struc: 202 a.a.

Protein chain

P9WI81  (PKNB_MYCTU) -  Serine/threonine-protein kinase PknB from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 626 a.a.

Struc: 189 a.a.

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1074/jbc.M116.731760

J Biol Chem 291:22961-22969 (2016)

PubMed id: 27601474

Structural and Genetic Analyses of the Mycobacterium tuberculosis Protein Kinase B Sensor Domain Identify a Potential Ligand-binding Site.

D.M.Prigozhin, K.G.Papavinasasundaram, C.E.Baer, K.C.Murphy, A.Moskaleva, T.Y.Chen, T.Alber, C.M.Sassetti.

ABSTRACT

No abstract given.