PDBsum entry 4z96

Hydrolase

PDB id: 4z96

Contents

Protein chain

523 a.a.

Ligands

ASN-LYS-GLY-LYS-GLY-LYS

GLY-LYS

PDB id:

4z96

Name:

Hydrolase

Title:

Crystal structure of dnmt1 in complex with usp7

Structure:

Ubiquitin carboxyl-terminal hydrolase 7. Chain: a. Fragment: unp residues 544-1067. Synonym: deubiquitinating enzyme 7,herpesvirus-associated ubiquitin- specific protease,ubiquitin thioesterase 7,ubiquitin-specific- processing protease 7. Engineered: yes. DNA (cytosine-5)-methyltransferase 1. Chain: c.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: usp7, hausp. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: dnmt1, aim, cxxc9, dnmt. Expression_system_taxid: 562

Resolution:

2.85Å R-factor: 0.204 R-free: 0.246

Authors:

Z.M.Zhang,J.Song

Key ref:

Z.M.Zhang and j.song Crystal structure of dnmt1 in complex with usp7. To be published, .

Date:

09-Apr-15 Release date: 12-Oct-16

Protein chain

Q93009  (UBP7_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase 7 from Homo sapiens

Seq: 1102 a.a.

Struc: 523 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).