PDBsum entry 4z7z

Hydrolase/DNA

PDB id: 4z7z

Contents

Protein chain

195 a.a.

DNA/RNA

Ligands

EDO

ACY

Waters ×209

PDB id:

4z7z

Name:

Hydrolase/DNA

Title:

Structure of the enzyme-product complex resulting from tdg action on a gt mismatch in the presence of excess base

Structure:

G/t mismatch-specific thymine DNA glycosylase. Chain: a. Fragment: unp residues 111-308. Synonym: thymine-DNA glycosylase,htdg. Engineered: yes. DNA (28-mer). Chain: c. Engineered: yes. DNA (28-mer).

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: tdg. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Resolution:

1.83Å R-factor: 0.195 R-free: 0.237

Authors:

E.Pozharski,S.S.Malik,A.C.Drohat

Key ref:

S.S.Malik et al. (2015). Thymine DNA glycosylase exhibits negligible affinity for nucleobases that it removes from DNA. Nucleic Acids Res, 43, 9541-9552. PubMed id: 26358812 DOI: 10.1093/nar/gkv890

Date:

08-Apr-15 Release date: 16-Sep-15

Protein chain

Q13569  (TDG_HUMAN) -  G/T mismatch-specific thymine DNA glycosylase from Homo sapiens

Seq: 410 a.a.

Struc: 195 a.a.

DNA/RNA chains

C-A-G-C-T-C-T-G-T-A-C-G-T-G-A-G-C-G-A-T-G-G-A-C-A-G-C-T 28 bases

A-G-C-T-G-T-C-C-A-T-C-G-C-T-C-A-ORP-G-T-A-C-A-G-A-G-C-T-G 28 bases

Enzyme reactions

• Enzyme class: E.C.3.2.2.29 - thymine-DNA glycosylase.

DOI no: 10.1093/nar/gkv890

Nucleic Acids Res 43:9541-9552 (2015)

PubMed id: 26358812

Thymine DNA glycosylase exhibits negligible affinity for nucleobases that it removes from DNA.

S.S.Malik, C.T.Coey, K.M.Varney, E.Pozharski, A.C.Drohat.

ABSTRACT

No abstract given.