PDBsum entry 4xo1

Protein binding

PDB id: 4xo1

Contents

Protein chain

60 a.a.

Waters ×44

PDB id:

4xo1

Name:

Protein binding

Title:

Crystal structure of se-met gnsa with double mutations

Structure:

Protein gnsa. Chain: a. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: gnsa, yccl, b4517, jw0976. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.196 R-free: 0.223

Authors:

L.Zhan,Z.Gao,Y.Dong

Key ref:

Y.Wei et al. (2015). Crystal structure of GnsA from Escherichia coli. Biochem Biophys Res Commun, 462, 1-7. PubMed id: 25839658 DOI: 10.1016/j.bbrc.2015.03.133

Date:

16-Jan-15 Release date: 29-Jul-15

Protein chain

P0AC92  (GNSA_ECOLI) -  Protein GnsA from Escherichia coli (strain K12)

Seq: 57 a.a.

Struc: 60 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DOI no: 10.1016/j.bbrc.2015.03.133

Biochem Biophys Res Commun 462:1-7 (2015)

PubMed id: 25839658

Crystal structure of GnsA from Escherichia coli.

Y.Wei, L.Zhan, Z.Gao, G.G.Privé, Y.Dong.

ABSTRACT

Escherichia Coli GnsA is a regulator of phosphatidylethanolamine synthesis and functions as a suppressor of both a secG null mutation and fabA6 mutations. GnsA may also be a toxin with the cognate antitoxin YmcE. Here we report the crystal structure of GnsA to 1.8 Å. GnsA forms a V shaped hairpin structure that is tightly associated into a homodimer. Our comprehensive structural study suggests that GnsA is structurally similar to an outer membrane protein, suggesting a function of protein binding.