 |
PDBsum entry 4pgo
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Unknown function
|
PDB id
|
|
|
|
4pgo
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Unknown function
|
|
|
Title:
|
|
Crystal structure of hypothetical protein pf0907 from pyrococcus furiosus solved by sulfur sad using swiss light source data
|
|
Structure:
|
|
Uncharacterized protein. Chain: a. Synonym: hypothetical protein pf0907. Engineered: yes
|
|
Source:
|
|
Pyrococcus furiosus. Organism_taxid: 186497. Strain: atcc 43587 / dsm 3638 / jcm 8422 / vc1. Gene: pf0907. Expressed in: escherichia coli. Expression_system_taxid: 562
|
|
Resolution:
|
|
|
2.30Å
|
R-factor:
|
0.189
|
R-free:
|
0.203
|
|
|
Authors:
|
|
T.Weinert,S.Waltersperger,V.Olieric,E.Panepucci,L.Chen,J.P.Rose, M.Wang,B.C.Wang,Southeast Collaboratory For Structural Genomics (Secsg)
|
|
Key ref:
|
|
T.Weinert
et al.
(2015).
Fast native-SAD phasing for routine macromolecular structure determination.
Nat Methods,
12,
131-133.
PubMed id:
DOI:
|
|
|
Date:
|
|
|
02-May-14
|
Release date:
|
10-Dec-14
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
Q8U2D2
(Q8U2D2_PYRFU) -
Elongation factor Tu-type domain-containing protein from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
|
|
|
|
Seq:
Struc:
|
|
|
|
108 a.a.
90 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
DOI no:
|
Nat Methods
12:131-133
(2015)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Fast native-SAD phasing for routine macromolecular structure determination.
|
|
T.Weinert,
V.Olieric,
S.Waltersperger,
E.Panepucci,
L.Chen,
H.Zhang,
D.Zhou,
J.Rose,
A.Ebihara,
S.Kuramitsu,
D.Li,
N.Howe,
G.Schnapp,
A.Pautsch,
K.Bargsten,
A.E.Prota,
P.Surana,
J.Kottur,
D.T.Nair,
F.Basilico,
V.Cecatiello,
S.Pasqualato,
A.Boland,
O.Weichenrieder,
B.C.Wang,
M.O.Steinmetz,
M.Caffrey,
M.Wang.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
We describe a data collection method that uses a single crystal to solve X-ray
structures by native SAD (single-wavelength anomalous diffraction). We solved
the structures of 11 real-life examples, including a human membrane protein, a
protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this
method. The data collection strategy is suitable for routine structure
determination and can be implemented at most macromolecular crystallography
synchrotron beamlines.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
