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PDBsum entry 4pgo
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Unknown function
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PDB id
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4pgo
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References listed in PDB file
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Key reference
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Title
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Fast native-Sad phasing for routine macromolecular structure determination.
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Authors
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T.Weinert,
V.Olieric,
S.Waltersperger,
E.Panepucci,
L.Chen,
H.Zhang,
D.Zhou,
J.Rose,
A.Ebihara,
S.Kuramitsu,
D.Li,
N.Howe,
G.Schnapp,
A.Pautsch,
K.Bargsten,
A.E.Prota,
P.Surana,
J.Kottur,
D.T.Nair,
F.Basilico,
V.Cecatiello,
S.Pasqualato,
A.Boland,
O.Weichenrieder,
B.C.Wang,
M.O.Steinmetz,
M.Caffrey,
M.Wang.
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Ref.
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Nat Methods, 2015,
12,
131-133.
[DOI no: ]
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PubMed id
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Abstract
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We describe a data collection method that uses a single crystal to solve X-ray
structures by native SAD (single-wavelength anomalous diffraction). We solved
the structures of 11 real-life examples, including a human membrane protein, a
protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this
method. The data collection strategy is suitable for routine structure
determination and can be implemented at most macromolecular crystallography
synchrotron beamlines.
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