PDBsum entry 4o2d

Ligase

PDB id: 4o2d

Contents

Protein chains

580 a.a.

515 a.a.

Ligands

ASP ×2

TRS ×4

Waters ×234

PDB id:

4o2d

Name:

Ligase

Title:

Crystal structure of aspartyl-tRNA synthetase from mycobacterium smegmatis with bound aspartic acid

Structure:

Aspartate--tRNA ligase. Chain: a, b. Synonym: aspartyl-tRNA synthetase, asprs. Engineered: yes

Source:

Mycobacterium smegmatis. Organism_taxid: 246196. Strain: atcc 700084/ mc(2)155. Gene: asps, msmeg_3003, msmei_2928. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.60Å R-factor: 0.198 R-free: 0.244

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

L.Baugh et al. (2015). Increasing the structural coverage of tuberculosis drug targets. Tuberculosis (edinb), 95, 142-148. PubMed id: 25613812 DOI: 10.1016/j.tube.2014.12.003

Date:

17-Dec-13 Release date: 22-Jan-14

Protein chain

A0QWN3  (SYDND_MYCS2) -  Aspartate--tRNA(Asp/Asn) ligase from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 598 a.a.

Struc: 580 a.a.*

Protein chain

A0QWN3  (SYDND_MYCS2) -  Aspartate--tRNA(Asp/Asn) ligase from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 598 a.a.

Struc: 515 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.6.1.1.23 - aspartate--tRNA(Asn) ligase.
• Reaction: tRNA(Asx) + L-aspartate + ATP = L-aspartyl-tRNA(Asx) + AMP + diphosphate

tRNA(Asx) + L-aspartate (Bound ligand (Het Group name = ASP) corresponds exactly) + ATP = L-aspartyl-tRNA(Asx) + AMP + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.tube.2014.12.003

Tuberculosis (edinb) 95:142-148 (2015)

PubMed id: 25613812

Increasing the structural coverage of tuberculosis drug targets.

L.Baugh, I.Phan, D.W.Begley, M.C.Clifton, B.Armour, D.M.Dranow, B.M.Taylor, M.M.Muruthi, J.Abendroth, J.W.Fairman, D.Fox, S.H.Dieterich, B.L.Staker, A.S.Gardberg, R.Choi, S.N.Hewitt, A.J.Napuli, J.Myers, L.K.Barrett, Y.Zhang, M.Ferrell, E.Mundt, K.Thompkins, N.Tran, S.Lyons-Abbott, A.Abramov, A.Sekar, D.Serbzhinskiy, D.Lorimer, G.W.Buchko, R.Stacy, L.J.Stewart, T.E.Edwards, W.C.Van Voorhis, P.J.Myler.

ABSTRACT

No abstract given.