PDBsum entry 4nx5

Lyase

PDB id: 4nx5

Contents

Protein chains

220 a.a.

Ligands

UP6 ×2

EDO

GOL ×2

Metals

_MG ×2

_CL ×4

Waters ×429

PDB id:

4nx5

Name:

Lyase

Title:

Crystal structure of orotidine 5'-monophosphate decarboxylase from methanobacterium thermoautotrophicum complexed with 6-azauridine 5'- monophosphate

Structure:

Orotidine 5'-phosphate decarboxylase. Chain: a, b. Fragment: r101p. Synonym: omp decarboxylase, ompdcase, ompdecase. Engineered: yes

Source:

Methanothermobacter thermautotrophicus. Organism_taxid: 145262. Gene: pyrf, mth_129. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.59Å R-factor: 0.148 R-free: 0.168

Authors:

A.A.Fedorov,E.V.Fedorov,K.K.Chan,J.A.Gerlt,S.C.Almo

Key ref:

A.A.Fedorov et al. Crystal structure of orotidine 5'-Monophosphate decarboxylase from methanobacterium thermoautotrophic complexed with 6-Azauridine 5'-Monophosphate. To be published, .

Date:

08-Dec-13 Release date: 25-Dec-13

Protein chains

O26232  (PYRF_METTH) -  Orotidine 5'-phosphate decarboxylase from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

Seq: 228 a.a.

Struc: 220 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.1.1.23 - orotidine-5'-phosphate decarboxylase.
• Pathway: Pyrimidine Biosynthesis
• Reaction: orotidine 5'-phosphate + H⁺ = UMP + CO2

orotidine 5'-phosphate + H(+) (Bound ligand (Het Group name = UP6) matches with 80.00% similarity) = UMP (Bound ligand (Het Group name = EDO) matches with 40.00% similarity) + CO2

Molecule diagrams generated from .mol files obtained from the KEGG ftp site