PDBsum entry 4mjh

Chaperone

PDB id: 4mjh

Contents

Protein chains

86 a.a.

80 a.a.

Ligands

ILE-THR-ILE-PRO-VAL-THR-PHE ×2

Waters ×1

PDB id:

4mjh

Name:

Chaperone

Title:

Human hsp27 core domain in complex with c-terminal peptide

Structure:

Heat shock protein beta-1. Chain: a, c. Fragment: core domain (unp residues 84-176). Synonym: hspb1, 28 kda heat shock protein, estrogen-regulated 24 kda protein, heat shock 27 kda protein, hsp 27, stress-responsive protein 27, srp27. Engineered: yes. Heat shock protein beta-1. Chain: b, d.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hsp27, hsp28, hspb1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 9606

Resolution:

2.60Å R-factor: 0.236 R-free: 0.266

Authors:

A.Laganowsky,D.Cascio,M.R.Sawaya,D.Eisenberg

Key ref:

G.K.Hochberg et al. (2014). The structured core domain of αB-crystallin can prevent amyloid fibrillation and associated toxicity. Proc Natl Acad Sci U S A, 111, E1562. PubMed id: 24711386 DOI: 10.1073/pnas.1322673111

Date:

03-Sep-13 Release date: 09-Apr-14

Protein chain

P04792  (HSPB1_HUMAN) -  Heat shock protein beta-1 from Homo sapiens

Seq: 205 a.a.

Struc: 86 a.a.

Protein chain

P04792  (HSPB1_HUMAN) -  Heat shock protein beta-1 from Homo sapiens

Seq: 205 a.a.

Struc: 80 a.a.

Enzyme reactions

• Enzyme class: Chains A, C: E.C.?

DOI no: 10.1073/pnas.1322673111

Proc Natl Acad Sci U S A 111:E1562 (2014)

PubMed id: 24711386

The structured core domain of αB-crystallin can prevent amyloid fibrillation and associated toxicity.

G.K.Hochberg, H.Ecroyd, C.Liu, D.Cox, D.Cascio, M.R.Sawaya, M.P.Collier, J.Stroud, J.A.Carver, A.J.Baldwin, C.V.Robinson, D.S.Eisenberg, J.L.Benesch, A.Laganowsky.

ABSTRACT

Mammalian small heat-shock proteins (sHSPs) are molecular chaperones that form polydisperse and dynamic complexes with target proteins, serving as a first line of defense in preventing their aggregation into either amorphous deposits or amyloid fibrils. Their apparently broad target specificity makes sHSPs attractive for investigating ways to tackle disorders of protein aggregation. The two most abundant sHSPs in human tissue are αB-crystallin (ABC) and HSP27; here we present high-resolution structures of their core domains (cABC, cHSP27), each in complex with a segment of their respective C-terminal regions. We find that both truncated proteins dimerize, and although this interface is labile in the case of cABC, in cHSP27 the dimer can be cross-linked by an intermonomer disulfide linkage. Using cHSP27 as a template, we have designed an equivalently locked cABC to enable us to investigate the functional role played by oligomerization, disordered N and C termini, subunit exchange, and variable dimer interfaces in ABC. We have assayed the ability of the different forms of ABC to prevent protein aggregation in vitro. Remarkably, we find that cABC has chaperone activity comparable to that of the full-length protein, even when monomer dissociation is restricted through disulfide linkage. Furthermore, cABC is a potent inhibitor of amyloid fibril formation and, by slowing the rate of its aggregation, effectively reduces the toxicity of amyloid-β peptide to cells. Overall we present a small chaperone unit together with its atomic coordinates that potentially enables the rational design of more effective chaperones and amyloid inhibitors.