PDBsum entry 4jkj

Hydrolase

PDB id: 4jkj

Contents

Protein chains

223 a.a.

Ligands

SO4 ×3

Waters ×95

PDB id:

4jkj

Name:

Hydrolase

Title:

Crystal structure of the s18y variant of ubiquitin carboxy-terminal hydrolase l1

Structure:

Ubiquitin carboxyl-terminal hydrolase isozyme l1. Chain: a, b. Synonym: uch-l1, neuron cytoplasmic protein 9.5, pgp 9.5, pgp9.5, ubiquitin thioesterase l1. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: uchl1. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

2.15Å R-factor: 0.198 R-free: 0.245

Authors:

C.W.Davies,D.Ringe,G.A.Petsko,C.Das

Key ref:

C.W.Davies et al. Crystal structure of the s18y variant of ubiquitin carboxy-Terminal hydrolase l1. To be published, .

Date:

09-Mar-13 Release date: 28-May-14

Protein chains

P09936  (UCHL1_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase isozyme L1 from Homo sapiens

Seq: 223 a.a.

Struc: 223 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).