PDBsum entry 4jgw

Signaling protein

PDB id: 4jgw

Contents

Protein chains

396 a.a.

Waters ×182

PDB id:

4jgw

Name:

Signaling protein

Title:

The conformation of a docking site for sh3 domains is pre-selected in the guanine nucleotide exchange factor rlf

Structure:

Ral guanine nucleotide dissociation stimulator-like 2. Chain: a, b. Fragment: unp residues 50-514. Synonym: ralgds-like 2, ralgds-like factor, ras-associated protein rab2l. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: rgl2, rab2l, rlf. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.30Å R-factor: 0.253 R-free: 0.288

Authors:

H.Rehmann,M.Popovic,A.J.Jakobi

Key ref:

M.Popovic et al. (2013). The guanine nucleotide exchange factor Rlf interacts with SH3 domain-containing proteins via a binding site with a preselected conformation. J Struct Biol, 183, 312-319. PubMed id: 23891840 DOI: 10.1016/j.jsb.2013.07.009

Date:

04-Mar-13 Release date: 11-Sep-13

Protein chains

Q61193  (RGL2_MOUSE) -  Ral guanine nucleotide dissociation stimulator-like 2 from Mus musculus

Seq: 778 a.a.

Struc: 396 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DOI no: 10.1016/j.jsb.2013.07.009

J Struct Biol 183:312-319 (2013)

PubMed id: 23891840

The guanine nucleotide exchange factor Rlf interacts with SH3 domain-containing proteins via a binding site with a preselected conformation.

M.Popovic, A.J.Jakobi, M.Rensen-de Leeuw, H.Rehmann.

ABSTRACT

Rlf is a guanine nucleotide exchange factor for the small G-proteins RalA and RalB and couples Ras- to Ral-signalling. Here the crystal structure of the catalytic module of Rlf consisting of a REM- and a CDC25-homology domain is determined. The structure is distinguished by an extended three stranded β-sheet called the flagpole. The flagpole is a conserved element in the RalGDS family of guanine nucleotide exchange factors and stabilises the orientation of the REM-domain relative to the CDC25-homology domain. A proline-rich sequence in the flagpole is unique to Rlf and several proteins that interact with this sequence by SH3 domains are identified. Conformational pre-selection results in a gain of affinity and contributes to the establishment of SH3 domain selectivity.