PDBsum entry 4imk

Immune system

PDB id: 4imk

Contents

Protein chains

229 a.a.

213 a.a.

Ligands

GOL ×2

SO4

Metals

_NA

Waters ×319

PDB id:

4imk

Name:

Immune system

Title:

Uncrossed fab binding to human angiopoietin 2

Structure:

Heavy chain. Chain: a, b. Engineered: yes. Light chain. Chain: c, d. Engineered: yes

Source:

Homo sapiens. Organism_taxid: 9606. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_taxid: 10029

Resolution:

2.20Å R-factor: 0.200 R-free: 0.226

Authors:

S.Fenn,C.Schiller,J.J.Griese,K.-P.Hopfner,H.Kettenberger

Key ref:

S.Fenn et al. (2013). Crystal structure of an anti-Ang2 CrossFab demonstrates complete structural and functional integrity of the variable domain. Plos One, 8, e61953. PubMed id: 23613981 DOI: 10.1371/journal.pone.0061953

Date:

03-Jan-13 Release date: 17-Apr-13

Protein chains

No UniProt id for this chain

Struc: 229 a.a.

Protein chains

No UniProt id for this chain

Struc: 213 a.a.

DOI no: 10.1371/journal.pone.0061953

Plos One 8:e61953 (2013)

PubMed id: 23613981

Crystal structure of an anti-Ang2 CrossFab demonstrates complete structural and functional integrity of the variable domain.

S.Fenn, C.B.Schiller, J.J.Griese, H.Duerr, S.Imhof-Jung, C.Gassner, J.Moelleken, J.T.Regula, W.Schaefer, M.Thomas, C.Klein, K.P.Hopfner, H.Kettenberger.

ABSTRACT

Bispecific antibodies are considered as a promising class of future biotherapeutic molecules. They comprise binding specificities for two different antigens, which may provide additive or synergistic modes of action. There is a wide variety of design alternatives for such bispecific antibodies, including the "CrossMab" format. CrossMabs contain a domain crossover in one of the antigen-binding (Fab) parts, together with the "knobs-and-holes" approach, to enforce the correct assembly of four different polypeptide chains into an IgG-like bispecific antibody. We determined the crystal structure of a hAng-2-binding Fab in its crossed and uncrossed form and show that CH1-CL-domain crossover does not induce significant perturbations of the structure and has no detectable influence on target binding.