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PDBsum entry 4imk
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Immune system
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PDB id
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4imk
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References listed in PDB file
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Key reference
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Title
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Crystal structure of an anti-Ang2 crossfab demonstrates complete structural and functional integrity of the variable domain.
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Authors
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S.Fenn,
C.B.Schiller,
J.J.Griese,
H.Duerr,
S.Imhof-Jung,
C.Gassner,
J.Moelleken,
J.T.Regula,
W.Schaefer,
M.Thomas,
C.Klein,
K.P.Hopfner,
H.Kettenberger.
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Ref.
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Plos One, 2013,
8,
e61953.
[DOI no: ]
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PubMed id
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Abstract
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Bispecific antibodies are considered as a promising class of future
biotherapeutic molecules. They comprise binding specificities for two different
antigens, which may provide additive or synergistic modes of action. There is a
wide variety of design alternatives for such bispecific antibodies, including
the "CrossMab" format. CrossMabs contain a domain crossover in one of
the antigen-binding (Fab) parts, together with the "knobs-and-holes"
approach, to enforce the correct assembly of four different polypeptide chains
into an IgG-like bispecific antibody. We determined the crystal structure of a
hAng-2-binding Fab in its crossed and uncrossed form and show that CH1-CL-domain
crossover does not induce significant perturbations of the structure and has no
detectable influence on target binding.
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