PDBsum entry 4f5c

Hydrolase/viral protein

PDB id: 4f5c

Contents

Protein chains

901 a.a.

146 a.a.

139 a.a.

Ligands

NAG-NAG ×3

NAG ×20

Metals

_ZN ×2

Waters ×4

PDB id:

4f5c

Name:

Hydrolase/viral protein

Title:

Crystal structure of the spike receptor binding domain of a porcine respiratory coronavirus in complex with the pig aminopeptidase n ectodomain

Structure:

Aminopeptidase n. Chain: a, b. Fragment: unp residues 36-963. Synonym: ap-n, papn, alanyl aminopeptidase, aminopeptidase m, ap-m, microsomal aminopeptidase, gp130. Engineered: yes. Prcv spike protein. Chain: e, f. Fragment: receptor binding domain (unp residues 17-445).

Source:

Sus scrofa. Pig. Organism_taxid: 9823. Gene: anpep. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: cho lec 3.2.8.1. Porcine respiratory coronavirus. Prcv.

Resolution:

3.20Å R-factor: 0.203 R-free: 0.245

Authors:

C.Santiago,J.Reguera,M.Gaurav,D.Ordono,L.Enjuanes,J.M.Casasnovas

Key ref:

J.Reguera et al. (2012). Structural bases of coronavirus attachment to host aminopeptidase N and its inhibition by neutralizing antibodies. Plos Pathog, 8, e1002859. PubMed id: 22876187

Date:

13-May-12 Release date: 22-Aug-12

Protein chains

P15145  (AMPN_PIG) -  Aminopeptidase N from Sus scrofa

Seq: 963 a.a.

Struc: 901 a.a.*

Protein chain

Q84852  (Q84852_9ALPC) -  Spike glycoprotein from Porcine respiratory coronavirus

Seq: 1225 a.a.

Struc: 146 a.a.*

Protein chain

Q84852  (Q84852_9ALPC) -  Spike glycoprotein from Porcine respiratory coronavirus

Seq: 1225 a.a.

Struc: 139 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.4.11.2 - membrane alanyl aminopeptidase.
• Reaction: Release of an N-terminal amino acid, Xaa-|-Xbb- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
• Cofactor: Zn(2+)

Plos Pathog 8:e1002859 (2012)

PubMed id: 22876187

Structural bases of coronavirus attachment to host aminopeptidase N and its inhibition by neutralizing antibodies.

J.Reguera, C.Santiago, G.Mudgal, D.Ordoño, L.Enjuanes, J.M.Casasnovas.

ABSTRACT

No abstract given.