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PDBsum entry 4dwc
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protein ligands metals Protein-protein interface(s) links
Viral protein PDB id
4dwc

 

 

 

 

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Contents
Protein chains
158 a.a.
Ligands
NAG-NAG-BMA-BMA-
MAN-MAN-BMA ×2
NAG-NAG ×4
NAG-NAG-FCA
NAG ×4
SO4 ×7
Metals
_ZN ×2
Waters ×44
PDB id:
4dwc
Name: Viral protein
Title: Crystal structure of the glycoprotein erns from the pestivirus bvdv-1 in complex with zn ions
Structure: E(rns) glycoprotein. Chain: a, b. Fragment: n-terminal fragment. Engineered: yes
Source: Bovine viral diarrhea virus. Bvdv. Organism_taxid: 268305. Strain: cp7. Gene: erns. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Resolution:
2.89Å     R-factor:   0.197     R-free:   0.227
Authors: T.Krey,F.Bontems,C.Vonrhein,M.-C.Vaney,G.Bricogne,T.Ruemenapf,F.A.Rey
Key ref: T.Krey et al. (2012). Crystal structure of the pestivirus envelope glycoprotein E(rns) and mechanistic analysis of its ribonuclease activity. Structure, 20, 862-873. PubMed id: 22579253
Date:
24-Feb-12     Release date:   23-May-12    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q96662  (POLG_BVDVC) -  Genome polyprotein from Bovine viral diarrhea virus (strain CP7)
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		3907 a.a.
158 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 1: E.C.2.7.7.48  - RNA-directed Rna polymerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
RNA(n)
 + ribonucleoside 5'-triphosphate
 = RNA(n+1)
 + diphosphate
   Enzyme class 2: E.C.3.4.21.113  - pestivirus NS3 polyprotein peptidase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
   Enzyme class 3: E.C.3.4.22.-  - ?????
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
   Enzyme class 4: E.C.3.6.1.15  - nucleoside-triphosphate phosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H+
ribonucleoside 5'-triphosphate
 + H2O
 = ribonucleoside 5'-diphosphate
 + phosphate
 + H(+)
   Enzyme class 5: E.C.3.6.4.13  - Rna helicase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + H2O = ADP + phosphate + H+
ATP
 + H2O
 = ADP
 + phosphate
 + H(+)
   Enzyme class 6: E.C.4.6.1.19  - ribonuclease T2.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: a ribonucleotidyl-ribonucleotide-RNA + H2O = a 3'-end 3'-phospho- ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + H+
RNA
 + H(2)O
 = (RNA fragment)-3'-nucleoside-3'-phosphate
 + 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Structure 20:862-873 (2012)
PubMed id: 22579253  
 
 
Crystal structure of the pestivirus envelope glycoprotein E(rns) and mechanistic analysis of its ribonuclease activity.
T.Krey, F.Bontems, C.Vonrhein, M.C.Vaney, G.Bricogne, T.Rümenapf, F.A.Rey.
 
  ABSTRACT  
 
No abstract given.

 

 

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