PDBsum entry 4duv

Hydrolase

PDB id: 4duv

Contents

Protein chains

1019 a.a.

Ligands

2DG ×4

BTB ×4

DMS ×90

Metals

_MG ×9

_NA ×16

Waters ×4018

PDB id:

4duv

Name:

Hydrolase

Title:

E. Coli (lacz) beta-galactosidase (g974a) 2-deoxy-galactosyl-enzyme and bis-tris complex

Structure:

Beta-galactosidase. Chain: a, b, c, d. Fragment: unp residues 10-1024. Synonym: beta-gal, lactase. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b0344, jw0335, lacz. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.159 R-free: 0.206

Authors:

R.W.Wheatley,S.Lo,L.J.Janzcewicz,M.L.Dugdale,R.E.Huber

Key ref:

R.W.Wheatley et al. The glucose acceptor site of lacz beta-Galactosidase synthesis of allolactose - The natural inducer of the operon. To be published, .

Date:

22-Feb-12 Release date: 03-Apr-13

Protein chains

P00722  (BGAL_ECOLI) -  Beta-galactosidase from Escherichia coli (strain K12)

Seq: 1024 a.a.

Struc: 1019 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.23 - beta-galactosidase.
• Reaction: Hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.