PDBsum entry 4dtb

Transferase

PDB id: 4dtb

Contents

Protein chains

296 a.a.

Ligands

GMP ×2

Metals

_CL

Waters ×197

PDB id:

4dtb

Name:

Transferase

Title:

Crystal structure of f95y aminoglycoside-2''-phosphotransferase type iva in complex with guanosine

Structure:

Aph(2'')-id. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Enterococcus casseliflavus. Organism_taxid: 37734. Gene: aph(2'')-id. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.10Å R-factor: 0.200 R-free: 0.250

Authors:

K.Shi,A.M.Berghuis

Key ref:

K.Shi and A.M.Berghuis (2012). Structural basis for dual nucleotide selectivity of aminoglycoside 2''-phosphotransferase IVa provides insight on determinants of nucleotide specificity of aminoglycoside kinases. J Biol Chem, 287, 13094-13102. PubMed id: 22371504

Date:

20-Feb-12 Release date: 07-Mar-12

Protein chains

O68183  (O68183_ENTCA) -  APH(2'')-Id from Enterococcus casseliflavus

Seq: 301 a.a.

Struc: 296 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.?

J Biol Chem 287:13094-13102 (2012)

PubMed id: 22371504

Structural basis for dual nucleotide selectivity of aminoglycoside 2''-phosphotransferase IVa provides insight on determinants of nucleotide specificity of aminoglycoside kinases.

K.Shi, A.M.Berghuis.

ABSTRACT

No abstract given.