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PDBsum entry 4dfc
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protein Protein-protein interface(s) links
Hydrolase/DNA binding protein PDB id
4dfc

 

 

 

 

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Contents
Protein chains
85 a.a.
121 a.a.
79 a.a.
PDB id:
4dfc
Name: Hydrolase/DNA binding protein
Title: Core uvra/trcf complex
Structure: Transcription-repair-coupling factor. Chain: a, c. Fragment: trcf-d2 domain, unp residues 127-213. Synonym: trcf, atp-dependent helicase mfd. Engineered: yes. Uvrabc system protein a. Chain: b, d. Fragment: unp residues 131-250. Synonym: uvra protein, excinuclease abc subunit a.
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b1114, escherichia coli, jw1100, mfd. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: b4058, dine, escherichia coli, jw4019, uvra.
Resolution:
2.80Å     R-factor:   0.240     R-free:   0.282
Authors: A.M.Deaconescu,N.Grigorieff
Key ref: A.M.Deaconescu et al. (2012). Nucleotide excision repair (NER) machinery recruitment by the transcription-repair coupling factor involves unmasking of a conserved intramolecular interface. Proc Natl Acad Sci U S A, 109, 3353-3358. PubMed id: 22331906
Date:
23-Jan-12     Release date:   02-May-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P30958  (MFD_ECOLI) -  Transcription-repair-coupling factor from Escherichia coli (strain K12)
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1148 a.a.
85 a.a.
Protein chains
Pfam   ArchSchema ?
P0A698  (UVRA_ECOLI) -  UvrABC system protein A from Escherichia coli (strain K12)
Seq:
Struc: 		 
Seq:
Struc: 		940 a.a.
121 a.a.*
Protein chain
Pfam   ArchSchema ?
P30958  (MFD_ECOLI) -  Transcription-repair-coupling factor from Escherichia coli (strain K12)
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1148 a.a.
79 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class 2: Chains A, C: E.C.3.6.4.-  - ?????
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
   Enzyme class 3: Chains B, D: E.C.3.6.1.3  - Deleted entry.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + H2O = ADP + phosphate
ATP
 + H(2)O
 = ADP
 + phosphate
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Proc Natl Acad Sci U S A 109:3353-3358 (2012)
PubMed id: 22331906  
 
 
Nucleotide excision repair (NER) machinery recruitment by the transcription-repair coupling factor involves unmasking of a conserved intramolecular interface.
A.M.Deaconescu, A.Sevostyanova, I.Artsimovitch, N.Grigorieff.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22960746 K.Howan, A.J.Smith, L.F.Westblade, N.Joly, W.Grange, S.Zorman, S.A.Darst, N.J.Savery, and T.R.Strick (2012).
Initiation of transcription-coupled repair characterized at single-molecule resolution.
  Nature, 490, 431-434.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.

 

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