 |
PDBsum entry 4b5c
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Lipid transport
|
PDB id
|
|
|
|
4b5c
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Lipid transport
|
|
|
Title:
|
|
Crystal structure of the peptidoglycan-associated lipoprotein from burkholderia pseudomallei
|
|
Structure:
|
|
Putative ompa family lipoprotein. Chain: a, b, c. Fragment: residues 36-170. Synonym: peptidoglycan-associated lipoprotein. Engineered: yes
|
|
Source:
|
|
Burkholderia pseudomallei. Organism_taxid: 28450. Strain: k96423. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: star.
|
|
Resolution:
|
|
|
2.30Å
|
R-factor:
|
0.209
|
R-free:
|
0.249
|
|
|
Authors:
|
|
L.J.Gourlay,C.Peri,O.Conchillo-Sole,M.Ferrer-Navarro,A.Gori,R.Longhi, D.Rinchai,G.Lertmemongkolchai,P.Lassaux,X.Daura,G.Colombo, M.Bolognesi
|
|
Key ref:
|
|
L.J.Gourlay
et al.
(2013).
Exploiting the Burkholderia pseudomallei acute phase antigen BPSL2765 for structure-based epitope discovery/design in structural vaccinology.
Chem Biol,
20,
1147-1156.
PubMed id:
DOI:
|
|
|
Date:
|
|
|
03-Aug-12
|
Release date:
|
14-Aug-13
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
Q63RA7
(Q63RA7_BURPS) -
Peptidoglycan-associated lipoprotein from Burkholderia pseudomallei (strain K96243)
|
|
|
|
Seq:
Struc:
|
|
|
|
170 a.a.
119 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
DOI no:
|
Chem Biol
20:1147-1156
(2013)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Exploiting the Burkholderia pseudomallei acute phase antigen BPSL2765 for structure-based epitope discovery/design in structural vaccinology.
|
|
L.J.Gourlay,
C.Peri,
M.Ferrer-Navarro,
O.Conchillo-Solé,
A.Gori,
D.Rinchai,
R.J.Thomas,
O.L.Champion,
S.L.Michell,
C.Kewcharoenwong,
A.Nithichanon,
P.Lassaux,
L.Perletti,
R.Longhi,
G.Lertmemongkolchai,
R.W.Titball,
X.Daura,
G.Colombo,
M.Bolognesi.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
We solved the crystal structure of Burkholderia pseudomallei acute phase antigen
BPSL2765 in the context of a structural vaccinology study, in the area of
melioidosis vaccine development. Based on the structure, we applied a recently
developed method for epitope design that combines computational epitope
predictions with in vitro mapping experiments and successfully identified a
consensus sequence within the antigen that, when engineered as a synthetic
peptide, was selectively immunorecognized to the same extent as the recombinant
protein in sera from melioidosis-affected subjects. Antibodies raised against
the consensus peptide were successfully tested in opsonization bacterial killing
experiments and antibody-dependent agglutination tests of B. pseudomallei. Our
strategy represents a step in the development of immunodiagnostics, in the
production of specific antibodies and in the optimization of antigens for
vaccine development, starting from structural and physicochemical principles.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
