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PDBsum entry 4b5c
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Lipid transport
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PDB id
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4b5c
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References listed in PDB file
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Key reference
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Title
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Exploiting the burkholderia pseudomallei acute phase antigen bpsl2765 for structure-Based epitope discovery/design in structural vaccinology.
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Authors
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L.J.Gourlay,
C.Peri,
M.Ferrer-Navarro,
O.Conchillo-Solé,
A.Gori,
D.Rinchai,
R.J.Thomas,
O.L.Champion,
S.L.Michell,
C.Kewcharoenwong,
A.Nithichanon,
P.Lassaux,
L.Perletti,
R.Longhi,
G.Lertmemongkolchai,
R.W.Titball,
X.Daura,
G.Colombo,
M.Bolognesi.
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Ref.
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Chem Biol, 2013,
20,
1147-1156.
[DOI no: ]
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PubMed id
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Abstract
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We solved the crystal structure of Burkholderia pseudomallei acute phase antigen
BPSL2765 in the context of a structural vaccinology study, in the area of
melioidosis vaccine development. Based on the structure, we applied a recently
developed method for epitope design that combines computational epitope
predictions with in vitro mapping experiments and successfully identified a
consensus sequence within the antigen that, when engineered as a synthetic
peptide, was selectively immunorecognized to the same extent as the recombinant
protein in sera from melioidosis-affected subjects. Antibodies raised against
the consensus peptide were successfully tested in opsonization bacterial killing
experiments and antibody-dependent agglutination tests of B. pseudomallei. Our
strategy represents a step in the development of immunodiagnostics, in the
production of specific antibodies and in the optimization of antigens for
vaccine development, starting from structural and physicochemical principles.
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