PDBsum entry 4zql

Ligase

PDB id: 4zql

Contents

Protein chains

177 a.a.

Ligands

SO4 ×2

DMS

4QH

1PE

Metals

_ZN ×4

Waters ×208

PDB id:

4zql

Name:

Ligase

Title:

Crystal structure of trim24 with 3,4-dimethoxy-n-(6-(4- methoxyphenoxy)-1,3-dimethyl-2-oxo-2,3-dihydro-1h-benzo[d]imidazol-5- yl)benzenesulfonamide inhibitor

Structure:

Transcription intermediary factor 1-alpha. Chain: a, b. Fragment: residues 791-972. Synonym: tif1-alpha,e3 ubiquitin-protein ligase trim24,ring finger protein 82,tripartite motif-containing protein 24. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: trim24, rnf82, tif1, tif1a. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.79Å R-factor: 0.161 R-free: 0.199

Authors:

C.Tallant,Structural Genomics Consortium (Sgc),P.G.K.Clark, L.C.C.Vieira,T.Krojer,G.Nunez-Alonso,S.Picaud,O.Fedorov,D.J.Dixon, F.Von Delft,C.H.Arrowsmith,A.M.Edwards,C.Bountra,P.E.Brennan,S.Knapp

Key ref:

P.G.Clark et al. Crystal structure of trim24 with 3,4-Dimethoxy-N-(6-(4-Methoxyphenoxy)-1,3-Dimethyl-2- 3-Dihydro-1h-Benzo[d]imidazol-5-Yl)benzenesulfonamide inhibitor. To be published, .

Date:

10-May-15 Release date: 17-Jun-15

Protein chains

O15164  (TIF1A_HUMAN) -  Transcription intermediary factor 1-alpha from Homo sapiens

Seq: 1050 a.a.

Struc: 177 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine