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PDBsum entry 4ram

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protein ligands metals Protein-protein interface(s) links
Hydrolase/antibiotic PDB id
4ram

 

 

 

 

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Contents
Protein chains
239 a.a.
Ligands
PNM ×2
Metals
_CL ×2
_ZN ×7
Waters ×370
PDB id:
4ram
Name: Hydrolase/antibiotic
Title: Crystal structure of new delhi metallo-beta-lactamase-1 mutant m67v complexed with hydrolyzed penicillin g
Structure: Beta-lactamase ndm-1. Chain: a, b. Synonym: ndm-1, metallo-beta-lactamase ndm-1. Engineered: yes. Mutation: yes
Source: Klebsiella pneumoniae. Organism_taxid: 573. Gene: blandm-1. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
1.50Å     R-factor:   0.145     R-free:   0.180
Authors: Y.Kim,C.Tesar,R.Jedrzejczak,G.Babnigg,J.Sacchettini,A.Joachimiak, Midwest Center For Structural Genomics (Mcsg),Structures Of Mtb Proteins Conferring Susceptibility To Known Mtb Inhibitors (Mtbi)
Key ref: Y.Kim et al. Crystal structure of new delhi metallo-Beta-Lactamase mutant m67V complexed with hydrolyzed penicillin g. To be published, .
Date:
10-Sep-14     Release date:   24-Sep-14    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
C7C422  (BLAN1_KLEPN) -  Metallo-beta-lactamase type 2 from Klebsiella pneumoniae
Seq:
Struc:
270 a.a.
239 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.5.2.6  - beta-lactamase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Penicillin Biosynthesis and Metabolism
      Reaction: a beta-lactam + H2O = a substituted beta-amino acid
      Cofactor: Zn(2+)

 

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