PDBsum entry 4gn2

Hydrolase

PDB id: 4gn2

Contents

Protein chain

240 a.a.

Waters ×281

PDB id:

4gn2

Name:

Hydrolase

Title:

Crystal structure of oxa-45, a class d beta-lactamase with extended spectrum activity

Structure:

Oxacillinase. Chain: a. Synonym: oxacillinase oxa45. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 287. Strain: 07-406. Gene: blaoxa45, oxa45. Expressed in: escherichia coli. Expression_system_taxid: 668369.

Resolution:

2.01Å R-factor: 0.169 R-free: 0.216

Authors:

J.D.Martin,X.L.Xiong,L.E.Catto,M.A.Toleman,T.R.Walsh,A.R.Clarke, J.Spencer

Key ref:

J.D.Martin et al. Structural and kinetic characterization of oxa-45, A beta-Lactamase with extended spectrum activity. To be published, .

Date:

16-Aug-12 Release date: 21-Aug-13

Protein chain

Q7WZC7  (Q7WZC7_PSEAI) -  Oxacillinase from Pseudomonas aeruginosa

Seq: 264 a.a.

Struc: 240 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)