PDBsum entry 4fn6

Transcription

PDB id: 4fn6

Contents

Protein chains

225 a.a.

Ligands

ACT ×9

GOL ×4

Waters ×81

PDB id:

4fn6

Name:

Transcription

Title:

Structural characterization of thiaminase type ii tena from staphylococcus aureus

Structure:

Thiaminase-2. Chain: a, b, c, d. Synonym: thiaminase ii. Engineered: yes

Source:

Staphylococcus aureus. Organism_taxid: 282458. Strain: mrsa252. Gene: tena, sar2183. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.69Å R-factor: 0.216 R-free: 0.258

Authors:

A.Begum,J.Drebes,M.Perbandt,C.Wrenger,C.Betzel

Key ref:

A.Begum et al. Structural characterization of thiaminase type ii ten staphylococcus aureus. To be published, .

Date:

19-Jun-12 Release date: 19-Dec-12

Protein chains

Q6GEY1  (TENA_STAAR) -  Aminopyrimidine aminohydrolase from Staphylococcus aureus (strain MRSA252)

Seq: 229 a.a.

Struc: 225 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.5.99.2 - aminopyrimidine aminohydrolase.
• Reaction:
  1. 4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5- hydroxymethyl-2-methylpyrimidine + NH4⁺
  2. thiamine + H2O = 5-(2-hydroxyethyl)-4-methylthiazole + 4-amino-5- hydroxymethyl-2-methylpyrimidine + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site