UniProt functional annotation for Q6GEY1

UniProt code: Q6GEY1.

Organism:		Staphylococcus aureus (strain MRSA252).
Taxonomy:		Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus.
Function:		Catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, a reaction that is part of a thiamine salvage pathway. Thus, catalyzes the conversion of 4- amino-5-aminomethyl-2-methylpyrimidine to 4-amino-5-hydroxymethyl-2- methylpyrimidine (HMP) (By similarity). Is also able to catalyze the hydrolytic cleavage of thiamine; however, this thiaminase activity may not be physiologically relevant. Therefore, is probably involved in the regeneration of the thiamine pyrimidine from thiamine degraded products present in the environment, rather than in thiamine degradation. {ECO:0000250|UniProtKB:P25052, ECO:0000269|PubMed:24311574, ECO:0000305}.
Catalytic activity:		Reaction=4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5- hydroxymethyl-2-methylpyrimidine + NH4(+); Xref=Rhea:RHEA:31799, ChEBI:CHEBI:15377, ChEBI:CHEBI:16892, ChEBI:CHEBI:28938, ChEBI:CHEBI:63416; EC=3.5.99.2; Evidence={ECO:0000250|UniProtKB:P25052};
Catalytic activity:		Reaction=H2O + thiamine = 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole + H(+); Xref=Rhea:RHEA:17509, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16892, ChEBI:CHEBI:17957, ChEBI:CHEBI:18385; EC=3.5.99.2; Evidence={ECO:0000269|PubMed:24311574};
Biophysicochemical properties:		Kinetic parameters: KM=1.40 mM for thiamine {ECO:0000269|PubMed:24311574}; Note=kcat is 4.07 ms(-1) for the hydrolysis of thiamine. {ECO:0000269|PubMed:24311574};
Pathway:		Cofactor biosynthesis; thiamine diphosphate biosynthesis. {ECO:0000250|UniProtKB:P25052}.
Subunit:		Homotetramer. {ECO:0000269|PubMed:24311574}.
Similarity:		Belongs to the TenA family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.