PDBsum entry 4dh7

Transferase/transferase inhibitor

PDB id: 4dh7

Contents

Protein chains

336 a.a.

20 a.a.

Ligands

ANP

Metals

_MG ×2

Waters ×454

PDB id:

4dh7

Name:

Transferase/transferase inhibitor

Title:

Low temperature x-ray structure of camp dependent protein kinase a catalytic subunit with high mg2+, amp-pnp and ip20'

Structure:

Camp-dependent protein kinase catalytic subunit alpha. Chain: a. Synonym: pka c-alpha. Engineered: yes. Camp-dependent protein kinase inhibitor alpha. Chain: i. Synonym: pki-alpha, camp-dependent protein kinase inhibitor, muscle/brain isoform. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: prkaca, pkaca. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: peptidic inhibitor was chemically synthesized

Resolution:

1.80Å R-factor: 0.179 R-free: 0.194

Authors:

A.Y.Kovalevsky,P.Langan

Key ref:

A.Y.Kovalevsky et al. (2012). Low- and room-temperature X-ray structures of protein kinase A ternary complexes shed new light on its activity. Acta Crystallogr D Biol Crystallogr, 68, 854-860. PubMed id: 22751671

Date:

27-Jan-12 Release date: 27-Jun-12

Protein chain

P05132  (KAPCA_MOUSE) -  cAMP-dependent protein kinase catalytic subunit alpha from Mus musculus

Seq: 351 a.a.

Struc: 336 a.a.*

Protein chain

P63248  (IPKA_MOUSE) -  cAMP-dependent protein kinase inhibitor alpha from Mus musculus

Seq: 76 a.a.

Struc: 20 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.11.11 - cAMP-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = ANP) matches with 81.25% similarity) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = ANP) matches with 81.25% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Acta Crystallogr D Biol Crystallogr 68:854-860 (2012)

PubMed id: 22751671

Low- and room-temperature X-ray structures of protein kinase A ternary complexes shed new light on its activity.

A.Y.Kovalevsky, H.Johnson, B.L.Hanson, M.J.Waltman, S.Z.Fisher, S.Taylor, P.Langan.

ABSTRACT

No abstract given.