PDBsum entry 4aim

Transferase/peptide

PDB id: 4aim

Contents

Protein chain

698 a.a.

Ligands

LYS-PRO-ARG-ARG-GLY-TRP-TRP-ARG

PO4

Waters ×2

PDB id:

4aim

Name:

Transferase/peptide

Title:

Crystal structure of c. Crescentus pnpase bound to rnase e recognition peptide

Structure:

Polyribonucleotide nucleotidyltransferase. Chain: a. Synonym: polynucleotide phosphorylase, pnpase. Engineered: yes. Ribonuclease, rne/rng family protein. Chain: c. Fragment: pnpase binding peptide - gww, residues 885-898. Synonym: rnase e. Engineered: yes

Source:

Caulobacter vibrioides. Organism_taxid: 190650. Strain: cb15. Atcc: 19089. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Caulobacter crescentus. Organism_taxid: 155892

Resolution:

3.30Å R-factor: 0.189 R-free: 0.260

Authors:

S.W.Hardwick,T.Gubbey,I.Hug,U.Jenal,B.F.Luisi

Key ref:

S.W.Hardwick et al. (2012). Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly. Open Biol, 2, 120028. PubMed id: 22724061

Date:

10-Feb-12 Release date: 18-Apr-12

Protein chain

Q9AC32  (PNP_CAUVC) -  Polyribonucleotide nucleotidyltransferase from Caulobacter vibrioides (strain ATCC 19089 / CB15)

Seq: 712 a.a.

Struc: 698 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.7.7.8 - polyribonucleotide nucleotidyltransferase.
• Reaction: RNA(n+1) + phosphate = RNA(n) + a ribonucleoside 5'-diphosphate

RNA(n+1) (Bound ligand (Het Group name = PO4) corresponds exactly) + phosphate = RNA(n) + ribonucleoside 5'-diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Open Biol 2:120028 (2012)

PubMed id: 22724061

Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly.

S.W.Hardwick, T.Gubbey, I.Hug, U.Jenal, B.F.Luisi.

ABSTRACT

No abstract given.