PDBsum entry 3w3d

Structural protein

PDB id: 3w3d

Contents

Protein chains

374 a.a.

260 a.a.

Ligands

NAG-NAG-BMA-BMA-MAN-MAN-BMA

ATP

Metals

_CA ×2

Waters ×383

PDB id:

3w3d

Name:

Structural protein

Title:

Crystal structure of smooth muscle g actin dnase i complex

Structure:

Actin, gamma-enteric smooth muscle. Chain: a. Synonym: alpha-actin-3, gamma-2-actin, smooth muscle gamma-actin. Deoxyribonuclease-1. Chain: b. Synonym: deoxyribonuclease i, dnase i. Ec: 3.1.21.1

Source:

Gallus gallus. Chicken. Organism_taxid: 9031. Other_details: gizzard. Bos taurus. Bovine. Organism_taxid: 9913. Other_details: pancreas

Resolution:

1.80Å R-factor: 0.196

Authors:

N.Sakabe,K.Sakabe,K.Sasaki,H.Kondo,M.Shimomur

Key ref:

K.Sasaki et al. (1993). Refined structure and solvent network of chicken gizz g-Actin dnase 1 complex at 1.8a resolution. Acta crystallogr ,Sect a, 49, c111.

Date:

20-Dec-12 Release date: 30-Jan-13

Protein chain

P63270  (ACTH_CHICK) -  Actin, gamma-enteric smooth muscle from Gallus gallus

Seq: 376 a.a.

Struc: 374 a.a.*

Protein chain

P00639  (DNAS1_BOVIN) -  Deoxyribonuclease-1 from Bos taurus

Seq: 282 a.a.

Struc: 260 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.3.6.4.- - ?????
• Enzyme class 2: Chain B: E.C.3.1.21.1 - deoxyribonuclease I.
• Reaction: Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.